Figure 3

Individual and simultaneous LCO-based quantification of curli and cellulose in longitudinal biofilm cultures. (a) Fluorescence of h-HTAA at 24 and 48 h in non-disrupted liquid cultures of 3934 wt (■), ΔbcsA (), ΔcsgA (▨) and ΔcsgD (□). (b) Spectra of h-HTAA in cultures at 24 h of 3934 wt (△) ΔbcsA (◊), ΔcsgA (□) and ΔcsgD (○), with emission read at 545 nm. (c) Fluorescence of h-FTAA at 24 and 48 h in non-disrupted liquid cultures of 3934 wt (■), ΔbcsA (), ΔcsgA (▨) and ΔcsgD (□). (d) Spectra of h-FTAA at 24 h of 3934 wt (△)ΔbcsA (◊), ΔcsgA (□) and ΔcsgD (○), with emission read at 545 nm. (e) Spectra of h-FTAA mixed with cellulose (5 mg/ml) with emission read at 545 nm. Data represent n: 1 of 3 with standard deviations shown in a and c. RFU, relative fluorescence units.