Figure 5
h-FTAA enables simultaneous, real-time detection of curli and cellulose in liquid Salmonella cultures. (a–d) Combined real-time recording of bacterial growth (left y-axis) measured by intensity of GFP (λEx 445 nm, λEm 510 nm), and h-FTAA staining extracellular curli (λEx 405 nm, λEm 556 nm) and cellulose (λEx 500 nm, λEm 600 nm; right y-axis) in liquid cultures of strains 3934 (a) wt, (b) ΔcsgD, (c) ΔcsgA and (d) ΔbcsA harbouring plasmid p2777. (e) Autofluorescence from LB medium only (□), as well as the combined background fluorescence from biofilm-forming, GFP-expressing bacteria in LB medium (○). (f) Combined real-time recording of bacterial growth, monitored by GFP expression (dashed line, left y-axis) from strain 3934 wt p2777, and appearance of cellulose detected by h-FTAA (right y-axis) in the absence (dotted line) and presence (solid line) of the cellulose-digesting enzyme cellulase.
