Figure 6
Visualisation of ECM components in biofilms formed by S. Enteritidis and S. Typhimurium in the presence of h-FTAA. (a–c) Fluorescence confocal microscopy of unfixed biofilm formed by S. Enteritidis strain 3934 wt p2777 at (a) lower and (b) higher magnification, as well as (c) S. Typhimurium strain 14028 ssaG:gfp+ during growth on inclined coverslips in medium supplemented with h-FTAA. Bacteria (green) and ECM (red) are detected at indicated wavelengths, representing the microscopes’ pre-defined detection settings for GFP and Cy3. (d–f) Fluorescence confocal microscopy images of 14028 ssaG:gfp+ (green) infected (d) CRL-4031 epithelial cells, and (e) RAW264.7 macrophage cell and in (f) sections of mouse livers stained with h-FTAA (red). Staining with Hoechst 33324 shows nuclei (blue) of each cell type. Single optical sections are shown. Scale bar=10 μm.
