Table 1 List of 27 genes analyzed in the retina by qRT-PCR for expression level

From: High dietary iron increases oxidative stress and radiosensitivity in the rat retina and vasculature after exposure to fractionated gamma radiation

 

CONTROL

RAD

IRON

IRON+RAD

Main effect

     

RAD

IRON

IRON+RAD

Apoptosis related

 ATG12 (autophagy-related protein 12)

84.58±33.00

108.41±28.60

52.56±8.83

67.55±14.14

P=0.007

P=0.08

 

 Bcl2 (B-cell lymphoma 2)

3.19±2.00

2.44±0.82

1.79±0.59

1.50±0.25

P=0.02

  

 Casp1 (caspase 1)

1.45±0.00

1.28±0.31

1.59±0.47

1.38±0.24

   

 Casp2 (caspase 2)

13.38±7.00

15.60±4.50

6.06±3.34

10.35±2.26

P=0.04

P=0.09

 

 Casp3 (caspase 3)

3.65±2.00

4.83±2.12

2.14±1.19

2.19±0.44

P=0.02

  

 CLU (clusterin)

728.83±254.00

789.68±206.88

601.21±196.35

589.38±193.74

   

 HiF1 (hypoxia-indicible factor)

265.43±193.00

443.87±331.98

183.64±45.33

185.27±113.26

P=0.09

  

Iron metabolism

 Cp1 (ceruloplasmin)

109.19±48.00

95.35±51.05

70.17±8.27

92.02±46.88

   

 FtH1 (Ferritin, Heavy)

2.58±0.58

2.89±0.53

2.36±0.39

2.82±1.31

   

 FtL1 (Ferritin, Light)

57.44±45.00

101.68±73.90

183.05±173.98

280.95±226.71

P=0.09

  

 Tf1 (transferrin 1)

3.21±1.14

3.26±2.32

2.40±7.34

3.55±2.04

   

Cellular growth

 Egr1 (early growth response factor)

28.26±33.00

20.44±23.24

28.41±32.77

15.02±8.19

   

 CFH (complement factor H)

61.74±26.00

73.44±17.29

34.92±4.25

71.75±30.97

 

P=0.02

 

 TrkA (tyrosine kinase receptor type 1)

13.10±7.00

7.46±4.48

6.41±3.26

10.21±5.72

  

P=0.10

 VEGF (vascular endothelial growth factor)

6.67±5.00

9.21±8.63

3.49±0.76

4.94±2.08

   

Eye growth and function

 Gfap (glial fibrillary acidic protein)

79.18±32.00

145.09±69.76

72.33±23.63

175.19±184.27

 

P=0.05

 

 Gndf (glial cell line derived neurotrophic factor)

1.40±0.00

1.69±0.86

1.69±0.52

1.38±0.24

   

 Opn4 (melanopsin pigment)

14.56±6.00

31.96±15.98

13.58±8.22

14.26±1.93

P=0.08

P=0.07

 

 Rho (rhodopsin pigment)

10.86±13.00

9.54±8.78

10.39±8.71

31.45±54.70

   

Cellular stress response

 Hmox1 (heme oxygenase 1)

7.08±2.39

4.67±1.32

8.08±1.31

14.70±2.20

  

P=0.001

 Nrf2 (nuclear factor like 2)

14.33±8.21

14.81±11.15

5.72±2.18

16.61±5.39

  

P=0.05

 HSF1 (heat shock factor)

50.57±15.93

53.66±17.22

25.50±4.36

28.23±7.22

P=0.001

  

 HSP27 (heat shock factor 27)

188.86±182.80

193.51±204.24

489.15±213.76

276.19±235.35

   

 HSP70 (heat shock factor 70)

1.40±0.36

1.76±0.85

1.59±0.47

1.45±0.24

P=0.02

  

 Sirt1 (Sirtuin 1)

26.21±12.16

31.42±10.57

15.14±4.68

18.66±3.54

   

 SOD2 (Mitochondrial superoxide dismutase)

168.39±83.41

152.27±113.83

100.78±22.94

230.01±177.47

   
  1. Abbreviations: ANOVA, analysis of variance; ECM, extracellular matrix; IRON, high-iron diet/no radiation; IRON+RAD, high-iron diet/radiation; qRT-PCR, quantitative reverse transcription PCR; RAD, adequate-iron diet/radiation.
  2. Expression level of 27 genes analyzed in the retina by qRT-PCR. The [(2−Δct)×1000] values for all genes investigated in the retina are listed. P values are based on a two-way ANOVA of the treatment effects calculated using Δct values.
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