Table 2 List of 84 genes analyzed in the vasculature by qRT-PCR for expression level
CONTROL | RAD | IRON | IRON+RAD | Main Effect | |||
|---|---|---|---|---|---|---|---|
RAD | IRON | IRON+RAD | |||||
Transmembrane | |||||||
Cd44 | 0.22±0.14 | 0.16±0.14 | 0.38±0.54 | 1.52±2.87 | P=0.06 | ||
Cdh2 (cadherin 2) | 0.94±0.54 | 1.94±2.96 | 1.13±0.72 | 0.86±0.59 | |||
Cdh3 (cadherin 3) | 1.48±1.00 | 1.16±1.05 | 1.54±1.03 | 5.28±8.58 | |||
Cdh4 (cadherin 4) | 5.68±2.33 | 8.01±5.24 | 5.42±1.91 | 9.96±3.44 | P=0.08 | ||
Ncam1 (neural cell adhesion molecule 1) | 17.75±6.58 | 11.31±6.54 | 18.29±13.02 | 17.99±19.39 | |||
Ncam2 (neural cell adhesion molecule 2) | 3.82±3.26 | 11.19±15.80 | 7.94±7.83 | 7.36±7.93 | |||
Pecam1 (platelet/endothelial cell adhesion molecule 1) | 64.83±46.27 | 48.01±17.11 | 57.65±31.41 | 61.31±35.57 | |||
Sele (selectin E) | 0.79±0.36 | 1.18±0.40 | 0.80±0.37 | 1.03±0.50 | P=0.07 | ||
Sell (selectin L) | 23.58±30.03 | 68.30±76.86 | 15.38±26.57 | 3.16±2.49 | |||
Selp (selectin P) | 2.77±1.78 | 4.16±4.21 | 2.08±1.04 | 1.78±1.11 | |||
Sgce (sarcoglycan, epsilon) | 33.81±12.87 | 31.92±14.23 | 36.11±10.18 | 35.19±12.49 | |||
Syt1 (synaptotagmin I) | 6.43±8.60 | 3.76±1.60 | 9.87±9.61 | 11.55±18.56 | |||
Cell–cell adhesion | |||||||
Cdh1 (cadherin 1) | 7.42±6.44 | 12.30±17.89 | 7.58±3.70 | 16.76±24.21 | |||
Icam1 (intercellular adhesion molecule 1) | 5.91±3.83 | 8.72±5.54 | 5.71±2.34 | 5.67±1.45 | |||
Vcam1 (vascular cell adhesion molecule 1) | 13.53±11.02 | 18.22±12.19 | 12.63±6.94 | 10.54±4.99 | |||
Cell–matrix adhesion | |||||||
Ctgf (connective tissue growth factor) | 76.28±31.49 | 127.38±131.22 | 74.40±25.76 | 140.60±40.54 | |||
Itga2 (integrin, alpha 2) | 1.94±0.92 | 1.75±0.45 | 2.87±3.16 | 10.35±18.19 | |||
Itga3 (integrin, alpha 3) | 9.68±4.60 | 9.13±4.10 | 10.46±4.94 | 17.99±11.27 | |||
Itga4 (integrin, alpha 4) | 22.22±28.97 | 25.06±18.35 | 9.07±6.93 | 10.33±6.54 | |||
Itga5 (integrin, alpha 5) | 44.29±33.89 | 35.03±33.56 | 27.99±15.51 | 31.69±23.39 | |||
Itgad (integrin, alpha D) | 1.53±2.00 | 2.82±2.95 | 0.67±0.42 | 0.91±0.20 | |||
Itgae (integrin, alpha E) | 12.48±4.00 | 13.06±6.31 | 10.41±3.36 | 13.83±10.93 | |||
Itgal (integrin, alpha L) | 26.38±44.72 | 13.54±12.92 | 5.95±5.34 | 6.16±3.72 | |||
Itgam (integrin, alpha M) | 0.76±0.39 | 0.84±0.64 | 0.73±0.31 | 0.87±0.41 | P=0.08 | ||
Itgav (integrin, alpha V) | 33.46±15.51 | 28.25±5.30 | 41.02±12.51 | 41.39±15.05 | |||
Itgb1 (integrin, beta 1) | 182.48±69.60 | 204.01±104.91 | 216.72±39.17 | 246.25±75.48 | |||
Itgb2 (integrin, beta 2) | 25.51±37.65 | 23.38±19.07 | 10.38±9.09 | 7.43±2.81 | P=0.09 | ||
Itgb3 (integrin, beta 3) | 8.07±3.45 | 21.37±36.73 | 8.28±5.25 | 23.14±22.54 | |||
Itgb4 (integrin, beta 4) | 7.30±5.62 | 8.29±3.71 | 11.40±6.87 | 15.05±20.22 | |||
Spp1 (secreted phosphoprotein 1) | 12.66±8.27 | 6.31±2.93 | 11.96±12.26 | 2.73±1.76 | P=0.07 | P=0.05 | |
Other adhesion molecules | |||||||
Catna1 (catenin, alpha 1) | 60.83±31.70 | 32.35±14.23 | 57.39±25.88 | 62.54±41.35 | |||
Ctnna2 (cadherin associated protein, alpha 2) | 0.38±0.52 | 0.66±0.47 | 0.87±1.18 | 0.70±1.11 | |||
Cntn1 (contactin 1) | 1.47±2.23 | 0.75±0.44 | 1.02±0.74 | 1.08±1.02 | |||
Ctnnb1 (cadherin associated protein, beta 1) | 71.13±29.93 | 63.09±17.46 | 78.80±11.97 | 119.46±45.09 | P=0.02 | ||
Emilin1 (eastin microfibril interfacer 1) | 4.63±1.56 | 11.11±17.14 | 4.51±2.28 | 7.38±4.06 | |||
Fn1 (fibronectin 1) | 44.46±21.46 | 36.06±15.89 | 43.93±18.43 | 29.98±17.90 | |||
Postn (osteoblast specific factor) | 112.89±34.70 | 159.26±90.42 | 155.67±83.42 | 190.23±158.34 | |||
Tgfbi (transforming growth factor beta) | 9.35±7.94 | 9.56±4.65 | 8.05±2.75 | 8.66±9.84 | |||
Thbs1 (thrombospondin 1) | 5.67±3.09 | 8.35±2.36 | 11.22±9.78 | 6.26±2.71 | |||
Thbs2 (thrombospondin 2) | 6.51±1.20 | 5.80±3.53 | 6.48±3.99 | 6.50±3.74 | |||
Vtn (vitronectin) | 9.94±6.06 | 6.69±4.68 | 11.10±5.35 | 9.96±6.79 | |||
Basement membrane | |||||||
Ecm1 (extracellular matrix protein 1) | 15.23±9.63 | 3.82±2.23 | 8.26±4.32 | 9.21±6.82 | |||
Entpd1 (ectonucleoside triphosphate diphosphohydrolase 1) | 4.57±2.71 | 4.04±0.89 | 4.96±1.05 | 3.71±1.47 | |||
Lama1 (laminin, alpha 1) | 1.11±0.65 | 1.36±1.32 | 0.69±0.30 | 1.78±1.56 | |||
Lama2 (laminin, alpha 2) | 51.06±16.27 | 53.77±37.49 | 58.78±28.85 | 70.56±24.38 | |||
Lama3 (laminin, alpha 3) | 4.51±2.05 | 4.70±3.99 | 5.13±2.18 | 8.19±7.82 | |||
Lamb2 (laminin, beta 2) | 130.86±43.36 | 193.55±242.87 | 105.34±32.78 | 142.70±59.77 | |||
Lamb3 (laminin, beta 3) | 2.13±0.91 | 5.96±9.21 | 3.36±3.60 | 11.55±18.73 | |||
Lamc1 (laminin, gamma 1) | 96.26±46.52 | 74.61±26.54 | 121.24±44.02 | 96.79±45.21 | |||
Sparc (secreted protein (osteonectin)) | 540.77±187.83 | 357.18±164.11 | 511.73±290.62 | 473.96±289.67 | |||
Collagens and ECM structure | |||||||
Col1a1 (collagen, type I) | 110.77±74.48 | 51.29±29.09 | 89.93±45.03 | 50.24±37.26 | P=0.04 | ||
Col2a1 (collagen, type II) | 0.33±0.41 | 0.35±0.34 | 0.22±0.14 | 0.55±0.37 | |||
Col3a1 (collagen, type III) | 522.01±176.67 | 362.30±162.60 | 508.44±157.25 | 356.68±136.37 | P=0.01 | ||
Col4a1 (collagen, type IV) | 198.74±114.41 | 124.41±60.43 | 219.44±150.38 | 176.99±143.98 | |||
Col4a2 (collagen, type IV) | 41.49±21.29 | 29.76±17.65 | 44.60±34.00 | 33.27±21.15 | |||
Col4a3 (collagen, type IV) | 18.87±10.43 | 14.30±9.88 | 13.87±5.27 | 22.03±16.94 | |||
Col5a1 (collagen, type V) | 18.47±11.59 | 9.60±3.94 | 14.67±5.35 | 9.98±5.03 | P=0.09 | ||
Col6a1 (collagen, type VI) | 14.03±9.57 | 5.43±2.63 | 10.23±7.81 | 7.34±4.63 | |||
Col8a1 (collagen, type VIII) | 0.28±0.15 | 0.40±0.48 | 0.16±0.09 | 0.42±0.27 | |||
Hapln1 (hyaluronan and proteoglycan link protein 1) | 0.34±0.23 | 0.95±1.65 | 0.30±0.27 | 0.72±0.86 | |||
ECM proteases | |||||||
Adamts1 (ADAM motif 1) | 32.03±18.77 | 28.05±29.75 | 24.30±13.22 | 22.80±9.50 | |||
Adamts2 (ADAM motif 2) | 16.84±9.01 | 25.29±21.95 | 15.98±9.67 | 83.46±71.23 | |||
Adamts5 (ADAM motif 5) | 28.48±11.95 | 29.39±16.68 | 38.95±13.44 | 34.34±13.01 | |||
Adamts8 (ADAM motif 8) | 5.81±2.53 | 21.08±22.16 | 6.73±6.29 | 22.41±13.47 | P=0.04 | ||
Mmp1a (matrix metallopeptidase 1a) | 0.16±0.11 | 0.74±1.31 | 0.09±0.04 | 0.36±0.14 | P=0.06 | ||
Mmp2 (matrix metallopeptidase 2) | 37.38±19.73 | 26.66±6.11 | 35.14±7.02 | 28.07±9.29 | |||
Mmp3 (matrix metallopeptidase 3) | 0.16±0.19 | 0.20±0.27 | 0.13±0.09 | 0.34±0.23 | P=0.07 | P=0.08 | |
Mmp7 (matrix metallopeptidase 7) | 0.67±0.36 | 1.99±2.86 | 1.06±0.97 | 1.41±1.04 | |||
Mmp8 (matrix metallopeptidase 8) | 0.76±0.61 | 0.53±0.23 | 0.36±0.21 | 0.58±0.25 | P=0.02 | ||
Mmp9 (matrix metallopeptidase 9) | 7.19±5.17 | 5.78±2.63 | 4.18±3.03 | 10.26±8.73 | |||
Mmp10 (matrix metallopeptidase 10) | 17.69±7.81 | 20.99±15.52 | 10.42±5.52 | 98.65±106.91 | P=0.05 | ||
Mmp11 (matrix metallopeptidase 11) | 61.56±32.37 | 23.82±12.73 | 20.40±17.17 | 56.75±43.71 | P=0.04 | ||
Mmp12 (matrix metallopeptidase 12) | 3.53±5.57 | 4.36±6.16 | 0.89±1.00 | 0.54±0.39 | |||
Mmp13 (matrix metallopeptidase 13) | 0.49±0.24 | 0.65±0.47 | 0.35±0.15 | 0.85±0.54 | |||
Mmp14 (matrix metallopeptidase 14) | 10.03±6.96 | 5.03±5.11 | 9.50±6.63 | 7.83±7.42 | |||
Mmp15 (matrix metallopeptidase 15) | 18.41±7.96 | 11.20±7.66 | 16.45±7.83 | 30.42±23.80 | P=0.07 | ||
Mmp16 (matrix metallopeptidase 16) | 0.96±0.56 | 0.32±0.17 | 0.54±0.36 | 0.26±0.30 | |||
ECM protease inhibitors | |||||||
Timp1 (TIMP metallopeptidase inhibitor 1) | 39.52±4.18 | 82.88±91.21 | 40.40±15.26 | 44.86±22.59 | |||
Timp2 (TIMP metallopeptidase inhibitor 2) | 205.76±55.90 | 151.46±26.83 | 211.01±40.58 | 179.26±50.71 | |||
Timp3 (TIMP metallopeptidase inhibitor 3) | 80.40±51.11 | 37.11±18.82 | 79.17±56.02 | 62.78±38.61 | |||
Other ECM molecules | |||||||
Fbln1 (fibulin 1) | 25.70±18.72 | 21.83±20.66 | 19.27±11.63 | 36.36±32.56 | |||
Spock1 (sparc/osteonectin 1) | 8.98±5.44 | 11.60±6.21 | 11.74±7.19 | 15.44±17.27 | |||
Tnc (tenascin C) | 6.24±6.89 | 3.05±1.44 | 4.52±3.37 | 2.95±1.39 | |||
Vcan (versican) | 20.72±9.59 | 32.96±40.49 | 19.53±8.65 | 19.74±10.78 | |||
