Table 2 CD4+ and CD8+ cell isolation results

From: Evaluation of techniques for performing cellular isolation and preservation during microgravity conditions

Sample

Cell type

Total cells

Viability

Viable cells

Total isolated DNA (ng)

Hopkins Ambient

All Cells

9×106

56%

5.22×106

NA

 

CD4+

1.5×106

63%

945,000

702

 

CD8+

1.1×106

72%

800,000

678

 

CD4−/CD8−

5.2×106

60%

3.2×106

1,890

Hopkins Frozen

All Cells

5.7×106

50%

2.85×106

NA

 

CD4+

850,000

63%

535,000

570

 

CD8+

420,000

73%

310,000

660

 

CD4−/CD8−

2.4×106

33%

780,000

330

ZeroG Frozen

All Cells

5.7×106

45%

2.56×106

NA

 

CD4+

840,000

59%

495,600

882

 

CD8+

990,000

45%

450,000

672

 

CD4−/CD8−

1.76×106

38%

660,000

1,038

  1. Abbreviation: NA, not applicable.