Figure 3
Growth under LSMMG increases expression of genes involved in lipid degradation and mycobactin synthesis but decreases expression of genes involved in mycolic acid synthesis. Genes involved in the designated categories lipid degradation (a), mycolic acid synthesis (b) and mycobactin synthesis (c) were identified using the mycobrowser website (http://mycobrowser.epfl.ch/) and the literature.38 The FPKMs (fragments per kilobase of transcript per million reads) for each gene in the 12 RNA samples were analyzed using Genesifter. Only genes with ⩾1.5-fold change in expression (P<0.05) were considered. The data were log transformed. The heat maps were generated showing expression in short (growth for ~40 h) and long (growth for 4 days ~35 h) exposure samples. Genes colored brown are discussed in the manuscript text.
