Figure 2 | npj Systems Biology and Applications

Figure 2

From: Combinatorial interventions inhibit TGFβ-driven epithelial-to-mesenchymal transition and support hybrid cellular phenotypes

Figure 2

Mesenchymal phenotype cells are enriched for combinatorial intervention target nodes predicted by the EMT network model. (a) Expression of epithelial (E-cadherin) and mesenchymal (vimentin) markers in HCC cell lines. (b) Immunofluorescent staining of EMT markers E-cadherin (green), vimentin (red), and nuclear stain DAPI (blue) in HCC cell lines Huh7, PLC/PRF/5 (Alexander), HepG2, and HLF. Expression of mRNA (c) by qRT-PCR and protein expression by immunoblot (d) of nodes whose paired knockout with SMAD is predicted by the EMT network model to inhibit TGFβ-driven EMT. (e) Epithelial-like HCC cells Huh7 were treated with TGFβ in serum free media for 48 h (1 ng/ml and 5 ng/ml), then mRNA expression of nodes whose paired knockout is predicted by the EMT network model to inhibit TGFβ-driven EMT was measured by qRT-PCR. EMT, Epithelial-to-mesenchymal transition, HCC, hepatocellular carcinoma, TGFβ, transforming growth factor beta, qRT-PCR, quantitative real-time PCR.

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