Figure 3
From: From START to FINISH: computational analysis of cell cycle control in budding yeast
Cdc14 release in strains carrying GAL-PDS1-dbΔ or GAL-SIC1-4A. These simulations are comparable to Figure 1 of ref. 42. (a) Control; same as Figure 2a. (b) In the mutant strain expressing a non-degradable version of Pds1 (securin), when Cdc20 is activated, Clb2 is only partially degraded, and the FEAR pathway (Pds1⊣ Esp1 ⊣ PPA2:Cdc55→Net1 ⊣ Cdc14) is inhibited. Consequently, Cdc14 remains sequestered in nucleolus, Cdh1:APC is not fully activated, and the cell cannot exit from mitosis. (c) Nonetheless, if cohesin molecules are cleaved by TEV protease, then the spindle position checkpoint can be turned off and the MEN pathway activated, promoting Cdc14 release and exit from mitosis, even though the FEAR pathway is blocked by non-degradable Pds1. (d,e) Cdc14 is not released in cells carrying either the GAL-SIC1-4A gene (encoding non-degradable Sic1) or the GAL-TEV construct, but (f) Cdc14 is released in cells carrying both GAL-SIC1-4A and GAL-TEV.
