Table 2 The three gap-filling steps

From: Network reconstruction and validation of the Snf1/AMPK pathway in baker’s yeast based on a comprehensive literature review

Reconstruction

Modification

Recon I

Unknown glucose-regulated step (US): stimulated by intracellular glucose, inhibited by NaCl, pH8, nitrogen limitation

 

If US active:

 

 SUMOylation of Snf1 is stimulated, deSUMOylation inhibited

 

 Elm1, Sak1, Tos3 are inhibited

 

 phosphorylation of Msn2 by Tpk1 is stimulated

Recon II

 phosphorylated Mig1 cannot be imported into the nucleus

 

 phosphorylated Snf1 cannot be exported from the nucleus

 

 NaCl prevents Snf1 import to nucleus

 

 phosphorylation of Gln3 induces nuclear export

Recon III

 Rsp5 antagonizing protease for Rod1

  1. The table indicates the modifications done in each of the three refinement steps.
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