Figure 2

Somatostatin (SST) mRNA+ cell density quantification in orbital frontal cortex (OFC) layer II. Representative photomicrographs showing (a) OFC layer II (magnification ×4; scale bar=200 μm; dotted line represents olfactory sulcus; quantification was performed in the gyrus rectus gray matter layer II), (b) orientation of the quantification frame (purple framed box; magnification ×10; scale bar=100 μm), (c) SST mRNA+ neurons in the quantification frame (magnification ×20; scale bar=20 μm), and (d) ×40 magnified view (scale bar=20 μm) showing SST mRNA+ neurons. Orange line indicates pial surface region (a), green line indicates OFC layer I region (a, b) and blue line indicates OFC layer II region (a, b). Red arrowheads (b–d) indicate SST mRNA+ neurons in OFC layer II.