Figure 2
(a) Locomotor activity of wild type (WT) and knockout (KO) mice; horizontal distances were measured for 60 min in an open field: WT: 44.0±8.3 m, n=13; αCaMKII-KO: 150.6±15.1 m, n=12 (unpaired two-tailed Student’s t-test; ***p<0.0001). (b) Extracellular dopamine levels in WT and αCaMKII-KO mice: Dopamine concentrations in the right dorsal striatum were collected by quantitative low-perfusion microdialysis and analyzed by HPLC–EC. WT: 11.54±2.31 nM, n=7; αCaMKII-KO: 20.15±2.92 nM, n=10, unpaired Student’s t-test; *p<0.05). (c) Tissue levels of dopamine: freshly dissected dorsal striata were homogenized and extracted before measurement by HPLC (n=14 per genotype; unpaired Student’s t-test; *p<0.05). (d) Densitometric analysis of western blots show no change in striatal TH expression between WT and KO animals; TH bands were normalized to tubulin; n=7; Student’s t-test, p>0.05.
