Figure 4
From: Involvement of Cholinergic System in Hyperactivity in Dopamine-Deficient Mice
Reduced AChex levels, ChAT gene expression, and ChAT protein levels in DD mice. (a) ChAT gene expression intensity in the area of the neostriatum, ventral striatum, and globus pallidus. The data are expressed as mean±SEM. *P<0.05, compared with wild-type mice (Student’s t-test). (b) Baseline AChex levels in the striatum in DD mice. The data are expressed as mean±SEM. *P<0.05, compared with wild-type mice (Student’s t-test). (c) Immunoblot analysis of ChAT protein expression in the striatum. Representative blots and data quantified against β-actin bands are shown as mean±SEM. *P<0.05, compared with wild-type mice (Student’s t-test). (d) Immunohistochemical analysis of ChAT. The two lower panels (C, D) are insets from the two upper panels (A, B). (A, C) Wild-type mice. (B, D) DD mice. Scale bars=200 μm (A, B) and 100 μm (C, D). Arrows indicate ChAT-positive cells.
