Figure 4
From: Dopamine D3 Receptor Is Necessary for Ethanol Consumption: An Approach with Buspirone
The selective TrKB antagonist, ANA-12 reverses ethanol intake of WT mice and induces D3 receptor downregulation but does not change ethanol intake of D3−/− mice. (a, b and c) Voluntary ethanol intake was measured every 24 h, for 34 days, in WT (n=30) and D3−/− (n=20) mice that had free access to water and ethanol solution (10%). At day 31, mice received daily i.p. injection of either vehicle (VEH), or ANA-12 at 0.5 mg/kg. (d, e) Drinking in the dark (DID) was measured, for 4 days, in WT (n=9) and D3−/− (n=9) mice that had limited access (2 h/day for 3 days and 4 h the 4th day) to ethanol solution (20%), daily injected with vehicle or ANA-12 1 h before the test. *p<0.05, **p<0.01, ***p<0.001 vs VEH, one-way ANOVA and Newman–Keuls post hoc test. (f) The abundance of transcripts of D3 receptor in striatum was assessed by quantitative RT-PCR in WT mice exposed to chronic voluntary ethanol intake. Mean fold changes are expressed relative to transcript levels in controls. The abundance of phosphorylated TrkB was assessed by immunoblot, in the striatum WT treated with ANA-12 and exposed to the voluntary ethanol intake. Bars show mean (± SEM). **p<0.01, ***p<0.001 vs vehicle. One-way ANOVA and Newman–Keuls post hoc test.
