Figure 5
From: Dopamine D3 Receptor Is Necessary for Ethanol Consumption: An Approach with Buspirone
Buspirone inhibits ethanol intake in WT mice both in the two bottle choice and DID paradigm. (a, b) Voluntary ethanol intake was measured every 24 h, for 44 days, in WT (n=20) and D3−/− (n=20) mice that had free access to water and ethanol solution (10%). Mice received for 14 days, from day 31, daily i.p. injection of either vehicle (VEH) or buspirone at 1 mg/kg. (c) Total fluid intake that was not changed by buspirone. *p<0.05, ***p<0.01 vs VEH. One-way ANOVA and Newman–Keuls post hoc test. (d) The dose ranging of buspirone (0.1, 1, 3, and 10 mg/kg) in WT mice exposed to the drinking in the dark (DID) paradigm. DID was measured, for 4 days, in WT (n=33) that had limited access (2 h/day for 3 days and 4 h the 4th day) to ethanol solution (20%). *p<0.05, **p<0.01 vs VEH. One-way ANOVA and Newman–Keuls post hoc test. (e) The effect of the selective 5-HT1A agonist, 8-OH-DPAT in DID paradigm. 8-OH-DPAT at 1 mg/kg did not change ethanol intake. (f) The action on 5-HT1A of 3 mg/kg buspirone was compared with 1 mg/kg 8-OH-DPAT by assessing the pharmacologically induced hypothermia. ***p<0.001 vs VEH. One-way ANOVA and Newman–Keuls post hoc test.
