Figure 5
Deleting PDE11A4 reduces ribosomal protein S6 phosphorylation, a biomarker of protein translation. (a) Relative to PDE11A WT littermates, PDE11A KO mice show no significant differences in expression of the ribosomal protein S6 (migrating ~32 kDa). (b) In contrast, PDE11A KO mice show a significant reduction in phosphorylation of S6 at serines 235/236 (p-235) in the ventral hippocampal formation (VHIPP) but not the dorsal hippocampal formation (DHIPP) whether pS6–235/236 is normalized by actin or (c) the total expression of S6. (d and e) The loss of pS6–235/236 appears to be selective as no difference was observed between PDE11A WT and KO mice in terms of pS6–240/244 (p-240). (f) PDE11A KO mice also show significantly reduced expression of an enzyme that phosphorylates S6 at serines 235/236 but not 240/244, namely ribosomal S6 kinase 2 (RSK2; migrating ~90 kDa). Relative to WT littermates, PDE11A KO mice show significantly reduced RSK2 expression in VHIPP but not DHIPP. (g) Representative western blots. VHIPP: n=18–19/genotype; DHIPP: n=24–26/genotype. *vs WT, P<0.05–0.001. Brightness and contrast adjusted for graphical clarity of blot images.
