Figure 2
Sex differences in locus coeruleus-μ-opioid receptor (LC-MOR) protein and mRNA. (a) Blots represent the MOR protein band (green) and β-actin band (red) as a loading control of LC-tissue punches from male (M) and female (F) rats. Note that the contrast was increased selectively around the molecular weight ladder to be able to visualize it. (b) Bars indicate the mean ratio of the integrated intensity of each band of MOR protein to the corresponding band of β-Actin as loading control from the same samples (n=11, each group). (c) Bars indicate relative quantification (RQ) of the MOR gene in the LC. Data are represented as the mean±SEM; (n=14, each group). GAPDH was used as an endogenous control. *p<0.05. A full color version of this figure is available at the Neuropsychopharmacology journal online.
