Figure 2
From: Activation of Ventral Tegmental Area 5-HT2C Receptors Reduces Incentive Motivation
5-HT2CRCRE:YFP VTA neurons co-express GABA and dopamine. (a) A 5-HT2CRCRE mouse line in which Cre recombinase is driven by a 5-HT2CR promoter was intercrossed with a ROSA26-stop-EYFP reporter mouse to generate a 5-HT2CRCRE:YFP line to facilitate the visualization of VTA 5-HT2CR expressing cells. (b) VTA 5-HT2CRCRE:YFP containing neurons (GFP-IF) have a greater co-expression with GAD67-IF compared with TH-IF (n=4 per group, p<0.001). (c) VTA 5-HT2CRCRE:YFP (GFP-IF; green) are co-localized (overlay, yellow) with endogenous 5-HT2CR-IF (red). (d) VTA 5-HT2CRCRE:YFP (GFP-IF; green) containing neurons and GAD67 (red) are co-localized (yellow). (e) VTA 5-HT2CRCRE:YFP (GFP-IF; green) containing neurons and TH (red) are co-localized (yellow). All the data are presented as mean±SEM. ∗∗∗p<0.001. Scale bar: 100 μm.
