Figure 5
Quantitative real-time PCR analysis of Crf, CrfR1, and CrfR2 expression in the amygdala, bed nucleus of the stria terminalis (BNST), and prefrontal cortex of stressed mice shows increased CrfR2 in male mice exposed to adolescent CUS. (a) Male and female mice exposed to adolescent or adult stress (Stress) have no fold change in Crf mRNA expression in the amygdala, BNST, or prefrontal cortex compared with controls (No Stress). Bars represent fold change measured by qRT-PCR and normalized to Tbp for Crf or HPRT for CrfR1 and CrfR2±SEM; n=9–13. (b) Stress had no effect on CrfR1 expression in the amygdala, BNST, or frontal cortex of male mice. Adult female mice exposed to adult CUS showed a significant decrease in CrfR1 expression in the amygdala compared with females exposed to adolescent CUS (#P<0.05), whereas no significant differences were found in other brain regions. (c) Males exposed to adolescent CUS showed a significant increase in CrfR2 expression in the amygdala compared with nonstressed controls (*P<0.05) and males exposed to adult CUS had a significant decrease in CrfR2 expression compared with males exposed to adolescent CUS (##P<0.001). No significant changes were found in other brain regions in CrfR2 expression in male mice or in female mice.
