Figure 4
16p11.2 df/+ mice displayed a learning and memory deficit in object location memory, which was reversed by R-baclofen treatment. R-baclofen (0.5 mg/ml), indicated by ‘+’, rescued the deficit in object location memory in 16p11.2 df/+ mice. (a) During the familiarization phase, no side bias was detected (WT, veh: t(21)=0.864; p=0.398, NS; df/+, veh: t(16)=1.31; p=0.208, NS; WT, R-baclofen: t(22)=1.97; p=0.062, NS; df/+, R-baclofen: t(19)=0.001, p=0.995, NS). (b) During the test phase, WT mice administered vehicle, indicated by ‘−’, spent significantly more time sniffing the object in the novel location (Nov) compared with time spent sniffing the object in the familiar location (Fam), while 16p11.2 df/+ mice administered vehicle did not exhibit significant object location memory (WT, veh: t(21)=2.34; p=0.029; df/+, veh: t(16)=0.305; p=0.764, NS). 16p11.2 df/+ mice administered R-baclofen spent significantly more time sniffing the object in the novel location compared with the object in the familiar location (df/+, R-baclofen t(19)=3.00, p<0.01). WT administered R-baclofen increased exploration of both objects and did not display the expected object location memory (WT, R-baclofen t(22)=0.641; p=0.528). *p<0.05, **p<0.01, R-baclofen vs vehicle within genotype.
