Figure 5
From: Dysfunction of Microglial STAT3 Alleviates Depressive Behavior via Neuron–Microglia Interactions
Depletion of microglial signal transducer and activator of transcription 3 (STAT3) enhances macrophage colony-stimulating factor (M-CSF)-mediated glutamatergic neurotransmission. (a) Representative traces of the miniature excitatory postsynaptic currents (mEPSCs) in the wild-type (WT) and knockout (KO) groups (n=10 and 9 neurons/group). Each asterisk indicates synaptic events. (b) Graphs for the cumulative probability of the mEPSC frequency; bar graphs for an average of frequency (1.2±0.27 Hz vs 2.14±0.5 Hz in the WT and KO mice, respectively). (c) Graphs for the cumulative probability of the mEPSC amplitudes; bar graphs for an average of amplitudes (17.185±1.306 pA vs 18.846±2.186 pA in the WT and KO mice, respectively). (d) Representative traces of the mEPSCs in the control and the M-CSF treatment groups (n=6 neurons/group). (e) Graphs for the cumulative probability of the mEPSC frequency; bar graphs for an average of frequency (1.18±0.19 Hz vs 2.22±0.412 Hz in the control and M-CSF treatment groups, respectively). (f) Graphs for the cumulative probability of the mEPSC amplitudes; bar graphs for an average of amplitudes (13.538±2.765 pA vs 11.651±2.620 pA in the control and M-CSF treatment groups, respectively). (g) Representative traces for paired pulse ratio measurement in WT and KO mice (n=6 and 5 neurons/group). (h) The Graph for paired pulse ratio at the 100 ms intervals (paired-pulse ratio (PPR): the ratio of EPSC2/EPSC1) (1.365±0.076 vs 0.935±0.058 in the WT and KO mice, respectively). (i) Dependence of PPR on interstimulus intervals at the 50, 100 and 150 ms (1.252±0.035 vs 1.013±0.038 at 50 ms, 1.365±0.076 vs 0.935±0.058 at 100 ms, 1.018±0.017 vs 0.96±0.03 at 150 ms in the WT and KO mice, respectively). (j) Representative images of Golgi staining in the WT and KO groups (n=5 mice/group). (k) Quantitative data for the number of dendritic spines (0.543±0.022 μm vs 0.556±0.015 μm in the WT and KO mice, n=17 and 23 neurons/group, respectively). Scale bar=40 μm. Scale bar of the enlarged image=20 μm. Data are means±SEM and *p<0.05, **p<0.01, and ***p<0.001.
