Abstract
The Barlow-Hall method for in vitro evolution is a simple alternative to methods such as DNA shuffling and is particularly applicable to predicting the evolution of target genes in nature. This detailed protocol includes the procedure itself and, as Appendices, describes particularly effective protocols for electroporation and for preparation of electrocompetent cells.
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Hall, B., Barlow, M. Barlow-Hall in vitro Evolution Protocol. Nat Prec (2008). https://doi.org/10.1038/npre.2008.1772.1
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DOI: https://doi.org/10.1038/npre.2008.1772.1
