Minimal Information About Sample Preparation for Phosphoproteomics

Abstract

This guideline describes parameters and conditions involved in phosphopeptide sample preparation. It covers from the description and preparation of the cells and tissues to the fractionation and specific enrichment of phosphopeptides for MS analysis. The guideline is prepared in order to easily cope with many of the experimental designs used in phosphoproteomic studies. The document is subdivided as follows:1. General features2. Sample processing3. Protein Purification/Fractionation4. Peptide Purification/Fractionation5. Phosphopeptide enrichment