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Gene expression

Grand masters of diversity

Nature Reviews Genetics volume 4, page 756 (2003)Cite this article

To test this theory, the authors investigated human and murine p53 , the product of which is a transcription factor that controls the expression of more than 50 downstream genes through direct binding to response elements (REs). These target genes are involved in a wide range of processes, including apoptosis, growth arrest, DNA repair and checkpoint responses.

The authors compared the in vivo transactivation capacity of wild type and mutant p53 using an isogenic yeast-based system that measures the activity of target REs upstream of the colour-marker reporter gene ADE2. Varying the levels of p53 expression allowed Resnick and Inga to identify mutants with altered binding affinities through changes in colony colour, which indicated differences in the patterns of target-gene activation and transcription.

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ORIGINAL RESEARCH PAPER

  1. Resnick, M. A. & Inga, A. Functional mutants of the sequence-specific transcription factor p53 and implications for master genes of diversity. Proc. Natl Acad. Sci. USA 100, 9934–9939 (2003)

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  1. Victoria Kitchener
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IARC TP53 Mutation Database

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Kitchener, V. Grand masters of diversity. Nat Rev Genet 4, 756 (2003). https://doi.org/10.1038/nrg1189

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  • DOI: https://doi.org/10.1038/nrg1189

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