Synthetic genome technology for yeast

Casci, Tanita

doi:10.1038/nrg3103

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Published: 18 October 2011

Genome engineering

Synthetic genome technology for yeast

Tanita Casci

Nature Reviews Genetics volume 12, page 742 (2011)Cite this article

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The authors have constructed a synthetic chromosome arm of the yeast Saccharomyces cerevisiae, laying the groundwork for creating a synthetic eukaryotic genome. A ∼90 kb stretch of the right arm of chromosome IX was designed in silico to remove unstable elements such as transposons and to facilitate genome manipulations. The synthetic sequence, which was swapped into the endogenous genomic region, also contains an inducible evolution system, SCRaMbLE (synthetic chromosome rearrangement and modification by loxP-mediated evolution), which triggers combinatorial rearrangements to allow the study of complex phenotypes.

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ORIGINAL RESEARCH PAPER

Dymond, J. S. et al. Synthetic chromosome arms function in yeast and generate phenotypic diversity by design. Nature 477, 471–476 (2011)
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