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An efficient new targeted resequencing method

Nature Reviews Genetics volume 12, page 805 (2011)Cite this article

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Resequencing of targeted genomic regions, such as exons, enables studies of genetic variation in large numbers of samples. This paper presents a new method called oligonucleotide-selective sequencing (OS-seq) in which oligonucleotides are immobilized on a substrate and used to capture sequences of interest before next-generation sequencing is performed on the same substrate. Additional preparation steps that are involved in other methods are avoided, thus increasing speed. To validate the method, OS-seq was successfully used to target the exons of ~350 cancer genes.

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ORIGINAL RESEARCH PAPER

  1. Myllykangas, S. et al. Efficient targeted resequencing of human germline and cancer genomes by oligonucleotide-selective sequencing. Nature Biotech. 23 Oct 2011 (doi:10.1038/nbt.1996)

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  1. Mary Muers
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Muers, M. An efficient new targeted resequencing method. Nat Rev Genet 12, 805 (2011). https://doi.org/10.1038/nrg3121

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