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DNase Hi-C — pitch-perfect chromatin mapping?

Nature Reviews Genetics volume 16, page 5 (2015)Cite this article

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Researchers at the University of Washington have developed a new high-throughput method to map chromatin interactions genome-wide. Named DNase Hi-C, this methodology uses DNase I for chromatin fragmentation, thus overcoming limitations on efficiency and resolution imposed on previous methods such as conventional Hi-C, which uses restriction enzymes for fragmentation. Among other experiments, the team validated their approach in two human cell lines by coupling DNase Hi-C with a targeted DNA sequence capture technology to map fine-scale three-dimensional chromatin architecture of 998 promoters of genes encoding long intergenic non-coding RNAs. Expression of lincRNAs was found to be tightly controlled via super-enhancers and Polycomb repressive complex 2.

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References

  1. Ma, W. et al. Fine-scale chromatin interaction maps reveal the cis-regulatory landscape of human lincRNA genes. Nature Methods http://dx.doi.org/10.1038/nmeth.3205 (2014)

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  1. Linda Koch
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Koch, L. DNase Hi-C — pitch-perfect chromatin mapping?. Nat Rev Genet 16, 5 (2015). https://doi.org/10.1038/nrg3881

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