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Chromatin

High-resolution 3D mapping of loops

Nature Reviews Genetics volume 16, page 68 (2015)Cite this article

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US researchers have mapped the spatial organization of the human genome at an unprecedentedly high resolution of 1 kb using in situ Hi-C, an updated Hi-C methodology that enables DNA proximity ligation in intact nuclei. This approach yielded maps comprising 5 Tb of sequence data, with more than 15 billion distinct contacts recorded, which is an order of magnitude greater than all previously published Hi-C data sets combined. The maps reveal conserved contact domains and the existence of ~10,000 chromatin loops, which often demarcate these contact domains. A great majority of loops were found to be anchored at a pair of convergent CTCF binding sites.

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References

  1. Rao, S. S. P. et al. A three-dimensional map of the human genome at kilobase resolution reveals principles of chromatin looping. Cell http://dx.doi.org/10.1016/j.cell.2014.11.021 (2014)

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  1. Linda Koch
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Koch, L. High-resolution 3D mapping of loops. Nat Rev Genet 16, 68 (2015). https://doi.org/10.1038/nrg3894

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