The three-dimensional structure of actively transcribing rotavirus particles reveals that the nascent mRNA transcripts generated within the core of the virion by endogenous transcriptase complexes are translocated through the intact capsid through a system of channels at the icosahedral five-fold axes.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Joklik, W.K. in: The Reoviridae (ed Joklik, W. K.) 1–7 (Plenum Press, New York. 1983).
Gottlieb, P., Strassman, J., Qiao, X., Frucht, A. & Mindich, L. J. Bacteriol. 172, 5774–5782 (1990).
Fields, B.N. in: Virology, (eds Fields, B. N. et al.) 1553–1555 (Raven Press, New York. 1996).
Gillies, S., Bullivant, S. & Bellamy, A.R. Science 174, 694–696 (1971).
Bartlett, N.M., Gillies, S.C., Bullivant, S. & Bellamy, A.R. J. Virol. 14, 315–326 (1974).
Yazaki, K. & Miura, K.-I. Virology 105, 467–479 (1980).
Dryden, K.A. et al. J. Cell Biol. 122, 1023–1041 (1993).
Prasad, B.V.V., Wang, G.J., Clerx, J.P.M. & Chiu, W. J. Mol. Biol. 199, 269–275 (1988).
Prasad, B.V.V., Rothnagel, R., Zeng, C.Q.-Y., Jakana, J., Lawton, J.A., Chiu, W. & Estes, M.K. Nature 382, 471–473 (1996).
Prasad, B.V.V., Yamaguchi, S. & Roy, P. J. Virol. 66, 2135–2142 (1992).
Kapikian, A.Z. & Chanock, R.M. in: Virology, (eds Fields, B. N. et al.) 1657–1708 (Raven Press, New York. 1996).
Estes, M.K. in Virology, (eds Fields, B. N. et al.) 1625–1655 (Raven Press, New York. 1996).
Dubochet, J. et al., Q. Rev. Biophys. 21, 129–228 (1988).
Cohen, J. J. Gen. Virol. 36, 395–402 (1977).
Cohen, J., LaPorte, J., Charpilienne, A. & Scherrer, R. Arch. Virol. 60, 177–186 (1979).
Sandino, A.M., Jashes, M., Faúndez, G. & Spencer, E. J. Virol. 60, 797–602 (1986).
Valenzuela, S. et al. J. Virol. 65, 3964–3967 (1991).
Zeng, C.Q.-Y., Wentz, M.J., Cohen, J., Estes, M.K. & Ramig, R.F. J. Virol. 70, 2736–2742 (1996).
Pizarro, J.L., Sandino, A.M., Pizarro, J.M., Fernández, J. & Spencer, E. J. Gen. Virol. 72, 325–332 (1991).
Liu, M., Mattion, N.M. & Estes, M.K. Virology 188, 77–84 (1992).
Horie, K., Wada, A. & Fukutome, H. J. Biochem. 90, 449–461 (1981).
Crowther, R.A. Phil. Trans. R. Soc. Lond. B 261, 221–230 (1971).
Fuller, S.D. Cell 48, 923–934 (1987).
Payne, C.C. & Mertens, P.P.C. in: The Reoviridae, (ed Joklik, W. K.) 1–7 (Plenum Press, New York. 1983).
Shaw, A.L., Samal, S.K., Subramanian, K. & Prasad, B.V.V. Structure 4, 957–967 (1996).
Mason, B.B., Graham, D.Y., & Estes, M.K. J. Virol. 33, 1111–1121 (1980).
Spencer, E. & Garcia, B.I. J. Virol. 52, 188–197 (1984).
Lawton, J.A. & Prasad, B.V.V. J. Struct. Biol. 116, 209–215 (1996).
Zhou, Z.H., Prasad, B.V.V., Jakana, J., Rixon, F.J. & Chiu, W. J. Mol Biol. 242, 456–469 (1994).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lawton, J., Estes, M. & Prasad, B. Three-dimensional visualization of mRNA release from actively transcribing rotavirus particles. Nat Struct Mol Biol 4, 118–121 (1997). https://doi.org/10.1038/nsb0297-118
Received:
Accepted:
Issue date:
DOI: https://doi.org/10.1038/nsb0297-118
This article is cited by
-
Structural insights into the coupling of virion assembly and rotavirus replication
Nature Reviews Microbiology (2012)
-
3.88 Å structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy
Nature (2008)