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Pushing back the limits of electron cryomicroscopy

Nature Structural Biology volume 4, pages 331–333 (1997)Cite this article

A convergence of technical advances in electron cryomicroscopy will allow visualization of biomolecular assemblies, without crystals, at resolutions below 10 Å. Recent cryomicroscopic structures of papillomavirus and hepatitis B virus capsid provide case studies.

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References

  1. Trus, B.L. et al. Nature Struct. Biol. 4, 411–418 (1997).

    Google Scholar 

  2. DeRosier, D.J. & Klug, A. Nature 217, 130–134 (1968).

    Article  CAS  Google Scholar 

  3. Crowther, R.A. Phil. Trans. Roy. Soc. Lond. B. 261, 221–230 (1971).

    Article  CAS  Google Scholar 

  4. Baker, T.S. & Cheng, H. J. Struct. Biol. 116, 120–130 (1996).

    Article  CAS  PubMed  Google Scholar 

  5. Böttcher, B., Wynne, S.A. & Crowther, R.A. Nature 386, 88–91 (1997).

    Article  PubMed  Google Scholar 

  6. Johnson, C.A., Weisenfeld, N.I., Trus, B.L., Conway, J.F., Martino, R.L. & Steven, A.C. Proc. Supercomputing 94, 550–559 (1994).

    Article  Google Scholar 

  7. Taylor, K.A. & Glaeser, R.M. Science 186, 1036–1037 (1974).

    Article  CAS  PubMed  Google Scholar 

  8. Dubochet, J. et al. Q. Rev. Biophys. 21, 129–228 (1988).

    Article  CAS  PubMed  Google Scholar 

  9. Avila-Sakar, A.J. & Chiu, W. Biophys. J. 70, 57–68 (1996).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Mitra, A.K., van Hoek, A. N., Wiener, M. C., Verkman, A. S. & Yeager, M. Nature Struct. Biol. 2, 726–729 (1995).

    Article  CAS  PubMed  Google Scholar 

  11. Jeng, T.W., Crowther, R.A., Stubbs, G. & Chiu, W. J. Mol. Biol. 205, 251–257 (1989).

    Article  CAS  PubMed  Google Scholar 

  12. Morgan, D.G., Owen, C., Melanson, L.A. & DeRosier, D.J. J. Mol. Biol. 249, 88–110 (1995).

    Article  CAS  PubMed  Google Scholar 

  13. Unwin, P.N.T. Nature 373, 37–43 (1995).

    Article  CAS  PubMed  Google Scholar 

  14. F., Zavodszky, P., Ishimura, M., Uedaira, H. & Namba, K. J. Mol. Biol. 251, 520–532 (1995).

    Article  Google Scholar 

  15. Zemlin, F. Ultramicroscopy 46, 25–32 (1992).

    Article  CAS  PubMed  Google Scholar 

  16. Zhou, Z.H. & Chiu, W. Ultramicroscopy 49, 407–416 (1993).

    Article  CAS  PubMed  Google Scholar 

  17. Conway, J.R. et al. Nature 386, 91–94 (1997).

    Article  CAS  PubMed  Google Scholar 

  18. Yan, Y., Stehle, T., Liddington, R.C., Zhao, H. & Harrison, S.C. Structure 4, 157–164 (1996).

    Article  CAS  PubMed  Google Scholar 

  19. Stehle, T., Gamblin, S.J., Yan, Y. & Harrison, S.C. Structure 4, 165–182 (1996).

    Article  CAS  PubMed  Google Scholar 

  20. Henderson, R. & Glaeser, R.M. Ultramicroscopy 16, 139–150 (1985).

    Article  CAS  Google Scholar 

  21. Henderson, R. Ultramicroscopy 46, 1–18 (1992).

    Article  CAS  PubMed  Google Scholar 

  22. Downing, K.H. Science 251, 53–59 (1991).

    Article  CAS  PubMed  Google Scholar 

  23. Jakubowski, U., Baumeister, W. & Glaeser, R.M. Ultramicroscopy 31, 351–356 (1989).

    Article  Google Scholar 

  24. Zhou, Z.H., Prasad, B.V.V., Jakana, J., Rixon, F. & Chiu, W. J. Mol. Biol. 242, 458–469 (1994).

    Article  Google Scholar 

  25. Grigorieff, N., Ceska, T.A., Downing, K.H., Baldwin, J.M. & Henderson, R. J. Mol. Biol. 259, 393–421 (1996).

    Article  CAS  PubMed  Google Scholar 

  26. K_ndt, W., Wang, D.N. & Fujiyoshi, Y. Nature 367, 614–621 (1994).

    Article  Google Scholar 

  27. Frank, J. et al. Nature 376, 441–444 (1995).

    Article  CAS  PubMed  Google Scholar 

  28. Stark, H. et al. Structure 3, 815–821 (1995).

    Article  CAS  PubMed  Google Scholar 

  29. Radermacher, M. et al. J. Cell Biol. 127, 411–423 (1994).

    Article  CAS  PubMed  Google Scholar 

  30. Orlova, E.V., Serysheva, I.I., van Heel, M., Hamilton, S. L. & Chiu, W. Nature Struct. Biol. 3, 547–552 (1996).

    Article  CAS  PubMed  Google Scholar 

  31. Akey, C.W. J. Mol. Biol. 248, 273–293 (1995).

    CAS  PubMed  Google Scholar 

  32. Roseman, A.M., Chen, S., White, H., Braig, K. & Saibil, H.R. Cell 87, 241–251 (1996).

    Article  CAS  PubMed  Google Scholar 

  33. Nitsch, M., Klumpp, M., Lupas, A. & Baumeister, W. J. Mol. Biol. 267, 142–149 (1997).

    Article  CAS  PubMed  Google Scholar 

  34. Rayment, I. et al. Science 261, 58–65 (1993).

    Article  CAS  PubMed  Google Scholar 

  35. Schmid, M.F., Agris, J., Jakana, J., Matsudaira, P. & Chiu, W. J. Cell Biol. 124, 341–350 (1994).

    Article  CAS  PubMed  Google Scholar 

  36. McGough, A., Way, M. & DeRosier, D. J. Cell Biol. 126, 433–443 (1994).

    Article  CAS  PubMed  Google Scholar 

  37. Whittaker, M. et al. Nature 378, 748–751 (1995).

    Article  CAS  PubMed  Google Scholar 

  38. Agrawal, R.K. et al. Science 271, 1000–1002 (1996).

    Article  CAS  PubMed  Google Scholar 

  39. Henderson, R. Quart. Rev. Biophys. 28, 171–193 (1995).

    Article  CAS  Google Scholar 

  40. Smith, T.J., Chase, E.S., Schmidt, T.J., Olson, N.H. & Baker, T.S. Nature 383, 350–354 (1996).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Stewart, P.L., Fuller, S.D. & Burnett, R.M. EMBO J. 12, 2589–2599 (1993).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. Baker, J.S. & Johnson, J.E. in Structural biology of viruses (eds. W. Chiu, R. M. Burnett and R. L. Garcea) 38–79 (Oxford University Press, New York, 1997).

    Google Scholar 

  43. Smith, T. J. et al. J. Virol. 67, 1148–1158 (1993).

    CAS  PubMed  PubMed Central  Google Scholar 

  44. Smith, T.S., Olson, N.H., Cheng, R.H., Chase, E.S. & Baker, T.S. Proc. Natl. Acad. Sci. USA 90, 7015–7018 (1993).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Max-Planck Institutfur Biochemie, D-82152, Martinsriedy, Germany

    Wah Chiu

  2. Verna and Mans McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX, 77030

    Michael F. Schmid

Authors
  1. Wah Chiu
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  2. Michael F. Schmid
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Chiu, W., Schmid, M. Pushing back the limits of electron cryomicroscopy. Nat Struct Mol Biol 4, 331–333 (1997). https://doi.org/10.1038/nsb0597-331

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