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Novel structural features of bovine papillomavirus capsid revealed by a three-dimensional reconstruction to 9 Å resolution

Nature Structural Biology volume 4, pages 413–420 (1997)Cite this article

Abstract

The three-dimensional structure of bovine papillomavirus has been determined to 9 Å resolution by reconstruction of high resolution, low dose cryo-electron micrographs of quench-f rozen virions. Although hexavalent and pentavalent capsomeres form star-shaped pentamers of the major capsid protein L1, they have distinct high-resolution structures. Most prominently, a 25 Å hole in the centre of hexavalent capsomeres is occluded in the pentavalent capsomeres. This raises the possibility that the L2 minor capsid protein is located in the centre of the pentavalent capsomeres. Inter-capsomere connections ∼10 Å in diameter were clearly resolved. These link adjacent capsomeres and are reminiscent of the helical connections that stabilize polyomavirus.

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Author information

Authors and Affiliations

  1. Computational Bioscience and Engineering Laboratory, Division of Computer Research and Technology, National Center for Research Resources, National Institutes of Health, Bethesda, Maryland, 20892-5624, USA

    Benes L. Trus

  2. Laboratory of Cellular Oncology, National Cancer Institute, National Center for Research Resources, National Institutes of Health, Bethesda, Maryland, 20892-5624, USA

    Richard B.S. Roden, Heather L. Greenstone & John T. Schiller

  3. Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Center for Research Resources, National Institutes of Health, Bethesda, Maryland, 20892-5624, USA

    Benes L. Trus & Frank P. Booy

  4. Biomedical Engineering and Instrumentation Program, National Center for Research Resources, National Institutes of Health, Bethesda, Maryland, 20892-5624, USA

    Michael Vrhel

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Trus, B., Roden, R., Greenstone, H. et al. Novel structural features of bovine papillomavirus capsid revealed by a three-dimensional reconstruction to 9 Å resolution. Nat Struct Mol Biol 4, 413–420 (1997). https://doi.org/10.1038/nsb0597-413

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