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Two structural configurations of the skeletal muscle calcium release channel

Nature Structural Biology volume 3, pages 547–552 (1996)Cite this article

Abstract

Here we present the determination of the three-dimensional structure of the skeletal muscle Ca2+-release channel in an open state using electron cryomicroscopy and angular reconstitution. In contrast to our reconstruction of the channel in its closed state, the density map of the channel driven towards its open state, by the presence of Ca2+ and ryanodine, features a central opening in the transmembrane region—the likely passageway for Ca2+ ions from the sarcoplasmic reticulum to the cytosol. The opening of the channel is associated with a 4° rotation of its transmembrane region with respect to its cytoplasmic region, and with significant mass translocations within the entire cytoplasmic region of the channel tetramer.

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Authors and Affiliations

  1. Verna and Marrs McLean Department of Biochemistry and The W.M. Keck Center for Computational Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030, USA

    Elena V. Orlova, Irina I. Serysheva & Wah Chiu

  2. Fritz Haber Institute of the Max Planck Society, Faradayweg 4-6, D-14195, Berlin, Germany

    Elena V. Orlova & Marin van Heel

  3. Department of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA

    Irina I. Serysheva, Susan L. Hamilton & Wah Chiu

  4. Department of Biochemistry, Imperial College of Science, Medicine and Technology, London, SW7 2AY, UK

    Marin van Heel

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  1. Elena V. Orlova
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  2. Irina I. Serysheva
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  4. Susan L. Hamilton
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  5. Wah Chiu
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Orlova, E., Serysheva, I., van Heel, M. et al. Two structural configurations of the skeletal muscle calcium release channel. Nat Struct Mol Biol 3, 547–552 (1996). https://doi.org/10.1038/nsb0696-547

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