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An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation

Nature Structural Biology volume 5, pages 787–792 (1998)Cite this article

An Erratum to this article was published on 01 October 1998

Abstract

Using a new procedure that combines electron-density correlation with biochemical information, we have fitted the crystal structure of the N-terminal actin-binding domain of human T-fimbrin to helical reconstructions of fimbrin-decorated actin filaments. The map locates the N-terminal calcium-binding domain and identifies actin-binding site residues on the two calponin-homology domains of fimbrin. Based on this map, we propose a model of a fimbrin crosslink in an actin bundle and its regulation by calcium.

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Figure 1: Modular organization of the calponin-homology (CH) domain superfamily.
Figure 2: A representative fit of the fimbrin (ABD1) crystal structure to the 3D reconstruction of the fimbrin–actin complex.
Figure 3: Space filling model of fimbrin (ABD1) with F-actin.
Figure 4: a, (upper panel) Translational scans along the axes of the N375 density.

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Acknowledgements

D.H. and N.V. wish to thank A. Brilliant for his valuable contributions to the graphics displayed in this paper. This work was supported by National Institutes of Health grants to D.DeR., W. L., R. C. (F. S. Fay), S.A. and P.M.

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Authors and Affiliations

  1. The W.M. Keck Institute for Cellular Visualization, Brandeis University, Waltham, 02254, Massachusetts, USA

    Dorit Hanein & David DeRosier

  2. Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, 02254, Massachusetts, USA

    Niels Volkmann

  3. Department of Biochemistry, Albert Einstein College of Medicine, Bronx, 10461, New York, USA

    Sharon Goldsmith & Steve Almo

  4. Department of Physiology, Boston University School of Medicine, Boston, 02118, Massachusetts, USA

    William Lehman

  5. Department of Cell Biology, University of Massachusetts Medical School, Worcester, 01655, Massachusetts, USA

    Roger Craig

  6. Department of Biology, Whitehead Institute for Biomedical Research, MIT, Cambridge, 02142, Massachusetts, USA

    Anne-Marie Michon & Paul Matsudaira

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Hanein, D., Volkmann, N., Goldsmith, S. et al. An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation. Nat Struct Mol Biol 5, 787–792 (1998). https://doi.org/10.1038/1828

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