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Watching protein folding unfold

Direct NMR observation of a transient folding intermediate provides new evidence for the importance of molten globules as general intermediates in protein folding.

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References

  1. Kim, P.S. & Baldwin, R.L. A. Rev. Biochem. 59, 631–660 (1990).

    Article  CAS  Google Scholar 

  2. Matthews, C.R. A. Rev. Biochem. 62, 653–683 (1993).

    Article  CAS  Google Scholar 

  3. Evans, P.A. & Radford, S.E. Curr. Opin. Struct. Biol. 4, 100–106 (1994).

    Article  CAS  Google Scholar 

  4. Roder, H. & Elöve, G.A. In Mechanisms of Protein Folding: Frontiers in Molecular Biology. (Ed. Pain, R.H.), 26–55 (Oxford University Press, New York; 1994).

    Google Scholar 

  5. Kuwajima, K. Proteins 6, 87 (1989).

    Article  CAS  Google Scholar 

  6. Ptitsyn, O.B. In Protein Folding. (Ed. Creighton, T.E.), 243–300 (W. H. Freeman & Co., New York; 1992).

    Google Scholar 

  7. Ptitsyn, O.B., Pain, R.H., Semisotnov, G.V., Zerovnik, E. & Razgulyaev, O.I. FEBS Lett. 262, 20 (1990).

    Article  CAS  Google Scholar 

  8. Balbach, J., Forge, V., van Nuland, N.A.J., Winder, S.L., Hore, P.J. & Dobson, C.M. Nature Struct. Biology 2, 866–870 (1995).

    Article  Google Scholar 

  9. Matthews, C.R. Meth. Enzymol. 154, 498–511 (1987).

    Article  CAS  Google Scholar 

  10. Matouschek, A., Kellis, J.T., Jr., Serrano, L., Bycroft, M. & Fersht, A.R. Nature 346, 440–445 (1990).

    Article  CAS  Google Scholar 

  11. Fersht, A.R. FEBS Lett. 325, 5–16 (1993).

    Article  CAS  Google Scholar 

  12. Wüthrich, K. NMR of Proteins and Nucleic Acids. (John Wiley & Sons, New York; 1986).

    Book  Google Scholar 

  13. Roder, H. Meth. Enzymol. 176, 446–473 (1989).

    Article  CAS  Google Scholar 

  14. Roder, H. & Wüthrich, K. Proteins 1, 34–42 (1986).

    Article  CAS  Google Scholar 

  15. Udgaonkar, J.B. & Baldwin, R.L. Nature 335, 694–699 (1988).

    Article  CAS  Google Scholar 

  16. Roder, H., Elöve, G.A. & Englander, S.W. Nature 335, 700–704 (1988).

    Article  CAS  Google Scholar 

  17. Udgaonkar, J.B. & Baldwin, R.L. Proc. natn. Acad. Sci. U.S.A. 87, 8197–8201 (1990).

    Article  CAS  Google Scholar 

  18. Elöve, G.A. & Roder, H. ACS Symp. Ser. 470, 50–63 (1991).

    Article  Google Scholar 

  19. Baldwin, R.L. & Roder, H. Curr. Biol. 1, 218–220 (1991).

    Article  CAS  Google Scholar 

  20. Englander, S.W. & Mayne, L. A. Rev. Biophys. Biomol. Struct. 21, 243–265 (1992).

    Article  CAS  Google Scholar 

  21. Baldwin, R.L. Curr. Opin. Struct. Biol. 3, 84–91 (1993).

    Article  CAS  Google Scholar 

  22. Blum, A.D., Smallcombe, S.H. & Baldwin, R.L. J. molec. Biol. 118, 305–316 (1978).

    Article  CAS  Google Scholar 

  23. Adler, M. & Scheraga, H.A. Biochemistry 27, 2471–2480 (1988).

    Article  CAS  Google Scholar 

  24. Kautz, R.A. & Fox, R.O. Protein Sci. 2, 851–858 (1993).

    Article  CAS  Google Scholar 

  25. Frieden, C., Hoeltzli, S.D. & Ropson, I.J. Protein Sci. 2, 2007–2014 (1993).

    Article  CAS  Google Scholar 

  26. Hoeltzli, S.D., Ropson, I.J. & Frieden, C. In Techniques in Protein Chemistry V. 455–465 (Academic Press, San Diego; 1994).

    Google Scholar 

  27. Marion, D., Ikura, M., Tschudin, R. & Bax, A. J. Magn. Reson. 85, 393–399 (1989).

    CAS  Google Scholar 

  28. Ruiz-Cabello, J., Vuister, G.W., Moonen, C.T.W., van Gelderen, P., Cohen, J.S. & van Zijl, P.C.M. J. Magn. Reson. 100, 282–302 (1992).

    CAS  Google Scholar 

  29. Kuwajima, K., Mitani, M. & Sugai, S. J. molec. Biol. 206, 547–561 (1989).

    Article  CAS  Google Scholar 

  30. Ikeguchi, M., Kuwajima, K., Mitani, M. & Sugai, S. Biochemistry 25, 6965–6972 (1986).

    Article  CAS  Google Scholar 

  31. Jennings, P.A. & Wright, P.E. Science 262, 892–895 (1993).

    Article  CAS  Google Scholar 

  32. Hughson, F.M., Wright, P.E. & Baldwin, R.L. Science 249, 1544–1548 (1990).

    Article  CAS  Google Scholar 

  33. Hughson, F.M., Barrick, D. & Baldwin, R.L. Biochemistry 30, 4113–4118 (1991).

    Article  CAS  Google Scholar 

  34. Baum, J., Dobson, C.M., Evans, P.A. & Hanley, C. Biochemistry 28, 7 (1989).

    Article  CAS  Google Scholar 

  35. Chyan, C.L., Wormald, C., Dobson, C.M., Evans, P.A. & Baum, J. Biochemistry 32, 5681–5691 (1993).

    Article  CAS  Google Scholar 

  36. Schulman, B.A., Redfield, C., Peng, Z.Y., Dobson, C.M. & Kim, P.S. J. molec. Biol. in the press.

  37. Ban, N. & Dobson, C. Nature struct. Biol. 2, 871–875 (1995).

    Article  Google Scholar 

  38. Jeng, M.-F., Englander, S.W., Elöve, G.A., Wand, A.J. & Roder, H. Biochemistry 29, 10433–10437 (1990).

    Article  CAS  Google Scholar 

  39. Marmorino, J.L. & Pielak, G.J. Biochemistry 34, 3140–3143 (1995).

    Article  CAS  Google Scholar 

  40. Dobson, C.M. Current Biology 4, 636–640 (1994).

    Article  CAS  Google Scholar 

  41. Alexandrescu, A.T., Evans, P.A., Pitkeatly, M., Baum, J. & Dobson, C.M. Biochemistry 32, 1707–1718 (1993).

    Article  CAS  Google Scholar 

  42. Ewbank, J.J. & Creighton, T.E. Nature 350, 518–520 (1991).

    Article  CAS  Google Scholar 

  43. Peng, Z.-y. & Kim, P.S. Biochemistry 33, 2136–2141 (1994).

    Article  CAS  Google Scholar 

  44. Ewbank, J.J. & Creighton, T.E. Biochemistry 32, 3677–3693 (1993).

    Article  CAS  Google Scholar 

  45. Wu, L.C., Peng, Z.-y. & Kim, P.S. Nature struct. Biology 2, 281–286 (1995).

    Article  CAS  Google Scholar 

  46. Sali, A., Shaknovich, E. & Karplus, M. Nature 369, 248–251 (1994).

    Article  CAS  Google Scholar 

  47. Gutin, A.M., Abkevich, V.I. & Shakhnovich, E.I. Biochemistry 34, 3066–3076 (1995).

    Article  CAS  Google Scholar 

  48. Acharya, K.R., Ren, J., Stuart, D.I. & Phillips, D.C. J. molec. Biol. 221, 571–581 (1991).

    Article  CAS  Google Scholar 

  49. Wolynes, P.G., Onuchic, J.N. & Thirumalai, D. Science 267, 1619–1620 (1995).

    Article  CAS  Google Scholar 

  50. Sosnick, T.R., Mayne, L., Hiller, R. & Englander, S.W. Nature struct. Biology 1, 149–156 (1994).

    Article  CAS  Google Scholar 

  51. Schmid, F.X. In Protein Folding. (Ed. Creighton, T.E.) 197–241 (W.H. Freeman & Co., New York; 1992).

    Google Scholar 

  52. Odefey, C., Mayr, L.M. & Schmid, F.X. J. molec. Biol. 245, 69–78 (1995).

    Article  CAS  Google Scholar 

  53. Creighton, T.E. Biochem. J. 270, 1–16 (1990).

    Article  CAS  Google Scholar 

  54. Weissman, J.S. & Kim, P.S. Proc. natn. Acad. Sci. U.S.A. 89, 9900–9904 (1992).

    Article  CAS  Google Scholar 

  55. Elöve, G.A., Bhuyan, A.K. & Roder, H. Biochemistry 33, 6925–6935 (1994).

    Article  Google Scholar 

  56. Wu, L., Laub, P.B., Elöve, G.A., Carey, J. & Roder, H. Biochemistry 32, 10271–10276 (1993).

    Article  CAS  Google Scholar 

  57. Dobson, C.M. Nature struct. Biology 2, 513–517 (1995).

    Article  CAS  Google Scholar 

  58. Jackson, S.E. & Fersht, A.R. Biochemistry 30, 10428–10435 (1991).

    Article  CAS  Google Scholar 

  59. Alexander, P., Orban, J. & Bryan, P. Biochemistry 31, 7243–7248 (1992).

    Article  CAS  Google Scholar 

  60. Viguera, A.R., Martúnez, J.C., Filimonov, V.V., Mateo, P.L. & Serrano, L. Biochemistry 33, 2142–2150 (1994).

    Article  CAS  Google Scholar 

  61. Kragelund, B.B., Robinson, C.V., Knudsen, J., Dobson, C.M. & Poulsen, F.M. Biochemistry 34, 7217–7224 (1995).

    Article  CAS  Google Scholar 

  62. Schindler, T., Herrler, M., Marahiel, M.A. & Schmid, F.X. Nature atruct. Biology 2, 663–673 (1995).

    Article  CAS  Google Scholar 

  63. Khorasanizadeh, S., Peters, I.D., Butt, T.R. & Roder, H. Biochemistry 32, 7054–7063 (1993).

    Article  CAS  Google Scholar 

  64. Houry, W.A., Rothwarf, D.M. & Scheraga, H.A. Biochemistry 33, 2516–2530 (1994).

    Article  CAS  Google Scholar 

  65. Jones, C.M. et al. Proc. natn. Acad. Sci. U.S.A. 90, 11860–11864 (1993).

    Article  CAS  Google Scholar 

  66. Regenfuss, P., Clegg, R.M., Fulwyler, M.J., Barrantes, F.J. & Jovin, T.M. Rev. Sci. Instrum. 56, 283–290 (1985).

    Article  CAS  Google Scholar 

  67. Kraulis, P.J. J. Appl. Cryst. 24, 946–950 (1991).

    Article  Google Scholar 

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Roder, H. Watching protein folding unfold. Nat Struct Mol Biol 2, 817–820 (1995). https://doi.org/10.1038/nsb1095-817

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