Abstract
Interaction of HIV-1 genomic RNA and human tRNA Lys 3 initiates viral reverse transcription. An adenosine-rich (A-rich) loop in HIV RNA mediates complex formation between tRNA and viral RNA. We have determined the structure of an A-rich loop oligonucleotide using nuclear magnetic resonance spectroscopy. The loop structure is stabilized by a non-canonical G–A pair and a U-turn motif, which leads to stacking of the conserved adenosines. The structure has similarity to the tRNA anticodon structure, and suggests possible mechanisms for its role in initiation of reverse transcription.
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Acknowledgements
The authors thank L. Elson for preparation of RNAs, S. Blanchard for preparation of labeled nucleotides and D. McKay for reading the manuscript. Supported by NIH Program Project grant in Structural Biology of AIDS.
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Puglisi, E., Puglisi, J. HIV-1 A-rich RNA loop mimics the tRNA anticodon structure. Nat Struct Mol Biol 5, 1033–1036 (1998). https://doi.org/10.1038/4141
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DOI: https://doi.org/10.1038/4141
