Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Structural basis for the accessory protein recruitment by the γ-adaptin ear domain

Nature Structural Biology volume 9, pages 527–531 (2002)Cite this article

Abstract

The adaptor proteins AP-1 and GGA regulate membrane traffic between the trans-Golgi network (TGN) and endosomes/lysosomes through ARF-regulated membrane association, recognition of sorting signals, and recruitment of clathrin and accessory proteins. The γ1-adaptin subunits of AP-1 and GGA possess homologous ear domains involved in the recruitment of accessory proteins, γ-synergin and Rabaptin-5. The crystal structure of the human γ1-adaptin ear domain consists solely of an immunoglobulin-like fold, unlike the α-adaptin ear domain. Structure-based mutational analyses reveal a binding site for the accessory proteins that is composed of conserved basic residues, indicating that the recruitment mechanism in γ1-adaptin and GGA is distinct from that in α-adaptin.

Access through your institution
Buy or subscribe

This is a preview of subscription content, access via your institution

Access options

Access through your institution

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Structure of the human γ1-ear domain.
Figure 2: Structure of the α-adaptin ear and β2-adaptin ear domains.
Figure 3: Conserved basic surface.
Figure 4: Pull-down assay.

Similar content being viewed by others

Accession codes

Accessions

GenBank/EMBL/DDBJ

Protein Data Bank

References

  1. Kirchhausen, T. Annu. Rev. Cell Dev. Biol. 15, 705–732 (1999).

    Article  CAS  Google Scholar 

  2. Boehm, M. & Bonifacino, J.S. Mol. Biol. Cell 12, 2907–2920 (2001).

    Article  CAS  Google Scholar 

  3. Page, L.J., Sowerby, P.J., Lui, W.W. & Robinson, M.S. J. Cell Biol. 146, 993–1004 (1999).

    Article  CAS  Google Scholar 

  4. Hirst, J. et al. J. Cell. Biol. 149, 67–80 (2000).

    Article  CAS  Google Scholar 

  5. Shiba, Y., Takatsu, H., Shin, H.W. & Nakayama, K. J. Biochem. 131, 327–336 (2002).

    Article  CAS  Google Scholar 

  6. Fernandez-Chacon, R., Alvarez de Toledo, G., Hammer, R.E. & Sudhof, T.C. J. Biol. Chem. 274, 32551–32554 (1999).

    Article  CAS  Google Scholar 

  7. Meyer, C. et al. EMBO J. 19, 2193–2203 (2000).

    Article  CAS  Google Scholar 

  8. Boman, A.L., Zhang, C., Zhu, X. & Kahn, R.A. Mol. Biol. Cell 11, 1241–1255 (2000).

    Article  CAS  Google Scholar 

  9. Dell'Angelica, E.C. et al. J. Cell Biol. 149, 81–94 (2000).

    Article  CAS  Google Scholar 

  10. Poussu, A., Lohi, O. & Lehto, V.P. J. Biol. Chem. 275, 7176–7183 (2000).

    Article  CAS  Google Scholar 

  11. Takatsu, H., Yoshino, K. & Nakayama, K. Biochem. Biophys. Res. Commun. 271, 719–725 (2000).

    Article  CAS  Google Scholar 

  12. Costaguta, G., Stefan, C.J., Bensen, E.S., Emr, S.D. & Payne, G.S. Mol. Biol. Cell 12, 1885–1896 (2001).

    Article  CAS  Google Scholar 

  13. Nielsen, M.S. et al. EMBO J. 20, 2180–2190 (2001).

    Article  CAS  Google Scholar 

  14. Puertollano, R., Aguilar, R.C., Gorshkova, I., Crouch, R.J. & Bonifacino, J.S. Science 292, 1712–1716 (2001).

    Article  CAS  Google Scholar 

  15. Takatsu, H., Katoh, Y., Shiba, Y. & Nakayama, K. J. Biol. Chem. 276, 28541–28545 (2001).

    Article  CAS  Google Scholar 

  16. Zhu, Y., Doray, B., Poussu, A., Lehto, V.P. & Kornfeld, S. Science 292, 1716–1718 (2001).

    Article  CAS  Google Scholar 

  17. Misra, S., Puertollano, R., Kato, Y., Bonifacino, J.S. & Hurley, J.H. Nature 415, 933–937 (2002).

    Article  CAS  Google Scholar 

  18. Shiba, T. et al. Nature 415, 937–941 (2002).

    Article  CAS  Google Scholar 

  19. Owen, D.J. et al. Cell 97, 805–815 (1999).

    Article  CAS  Google Scholar 

  20. Traub, L.M., Downs, M.A., Westrich, J.L. & Fremont, D.H. Proc. Natl. Acad. Sci. USA 96, 8907–8912 (1999).

    Article  CAS  Google Scholar 

  21. Owen, D.J., Vallis, Y., Pearse, B.M., McMahon, H.T. & Evans, P.R. EMBO J. 19, 4216–4227 (2000).

    Article  CAS  Google Scholar 

  22. Leslie, A.G.W. Crystallographic Computing (Oxford University Press, Oxford; 1990).

    Google Scholar 

  23. Collaborative Computational Project, Number 4. Acta Crystallogr. D 50, 760–763 (1994).

  24. Terwilliger, T.C. & Berendzen, J. Acta Crystallogr. D 55, 849–861 (1999).

    Article  CAS  Google Scholar 

  25. Terwilliger, T.C. Acta Crystallogr. D 56, 965–972 (2000).

    Article  CAS  Google Scholar 

  26. Perrakis, A., Morris, R. & Lamzin, V.S. Nature Struct. Biol. 6, 458–463 (1999).

    Article  CAS  Google Scholar 

  27. Jones, T.A., Zou, J.Y., Cowan, S.W. & Kjeldgaard, M. Acta Crystallogr. A 47, 110–119 (1991).

    Article  Google Scholar 

  28. Laskowski, R.A., MacArthur, M.W., Moss, D.S. & Thornton, J.M. J. Appl. Crystallogr. 26, 283–291 (1993).

    Article  CAS  Google Scholar 

  29. Kraulis, P.J. J. Appl. Crystallogr. 24, 946–950 (1991).

    Article  Google Scholar 

  30. Nicholls, A., Sharp, K.A. & Honig, B. Proteins 11, 281–296 (1991).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, from the Japan Society for Promotion of Science (fellowship to H.T.) and from the University of Tsukuba Research Projects.

Author information

Author notes

  1. Yoko Shiba & Kazuhisa Nakayama

    Present address: Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa, 920-0934, Japan

Authors and Affiliations

  1. Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), Tsukuba, 305-0801, Ibaraki, Japan

    Terukazu Nogi, Masato Kawasaki, Tomoo Shiba, Naohiro Matsugaki, Noriyuki Igarashi, Mamoru Suzuki, Ryuichi Kato & Soichi Wakatsuki

  2. Institute of Biological Sciences and Gene Research Center, University of Tsukuba, Tsukuba, 305-8572, Ibaraki, Japan

    Yoko Shiba, Hiroyuki Takatsu & Kazuhisa Nakayama

  3. Foundation for Advancement of International Science (FAIS), Tsukuba, 305-0062, Ibaraki, Japan

    Tomoo Shiba

Authors
  1. Terukazu Nogi
    View author publications

    Search author on:PubMed Google Scholar

  2. Yoko Shiba
    View author publications

    Search author on:PubMed Google Scholar

  3. Masato Kawasaki
    View author publications

    Search author on:PubMed Google Scholar

  4. Tomoo Shiba
    View author publications

    Search author on:PubMed Google Scholar

  5. Naohiro Matsugaki
    View author publications

    Search author on:PubMed Google Scholar

  6. Noriyuki Igarashi
    View author publications

    Search author on:PubMed Google Scholar

  7. Mamoru Suzuki
    View author publications

    Search author on:PubMed Google Scholar

  8. Ryuichi Kato
    View author publications

    Search author on:PubMed Google Scholar

  9. Hiroyuki Takatsu
    View author publications

    Search author on:PubMed Google Scholar

  10. Kazuhisa Nakayama
    View author publications

    Search author on:PubMed Google Scholar

  11. Soichi Wakatsuki
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Soichi Wakatsuki.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nogi, T., Shiba, Y., Kawasaki, M. et al. Structural basis for the accessory protein recruitment by the γ-adaptin ear domain. Nat Struct Mol Biol 9, 527–531 (2002). https://doi.org/10.1038/nsb808

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue date:

  • DOI: https://doi.org/10.1038/nsb808

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing