Supplementary Figure 1: Biochemical characterization.
From: Structure of the p300 catalytic core and implications for chromatin targeting and HAT regulation
(a) Mass spectrometry analysis using electrospray ionization (ESI) of the p300 'core' before, and after (b) deacetylation by SIRT2. Inset shows a Western blot of p300 deacetylation by SIRT2. Levels of acetylation were detected by western blot using an anti–acetyllysine antibody. Incubation times in hours (h) are indicated above the gel. After overnight (o/n) incubation, SIRT2 was able to completely deacetylate the p300 'core'. (c) HAT activity was measured with a variant in which the AIL was replaced with a 5 residue linker (Δ1520–1581). Substrate was a peptide derived from histone H3 (1–20) ARTKQTQRKSTGGKAPRKQL.
