Supplementary Figure 4: The THO mutant hpr1 shows increased telomeric RNA-DNA hybrids and increased rates of telomeric recombination

(a) hpr1 shows increased telomeric RNA-DNA hybrids. ChIP was carried out as described in Fig. 1a. The values are represented as % input of telomeric DNA recovered relative to wild type (set to 1) and are the means of 5 biological replicates, error bars depict ± s.e.m. P values were derived from a two-tailed one-sample Student's t-test. (b) Deletion of HPR1 accelerates senescence in HR deficient telomerase mutants. In the presence of RAD52 the viability loss was alleviated, suggesting HR-mediated compensation. Liquid senescence assays were performed on the indicated strains. Curves represent the average value for 6 biological replicates ± s.e.m. (c) hpr1 shows increased rates of telomeric recombination. 1L Telomere-PCR was performed on genomic DNA extracted from the two indicated clones, which were derived form the same tetrad. 1L Telomere-PCR products were cloned and sequenced as described in Fig. 2a and % of diverged telomeres was calculated (est2 n = 48, PD 30; est2 hpr1 n = 39; PD 26).