Supplementary Figure 1: Telomeric RNA-DNA hybrids are regulated by RNase H enzymes
From: Telomeric RNA-DNA hybrids affect telomere-length dynamics and senescence
RNA-DNA hybrids exist at telomeres and accumulate in RNase H mutants. The identical ChIP as described in Fig. 1a, however quantified showing % input on the y axis. Values are represented as % input of telomeric DNA recovered and are the means of seven (+ab) and five (-ab) biological replicates, error bars depict ± s.e.m.P values were derived from two-tailed Student's t-tests (NS = not significant). (b) The RNA-DNA hybrid signal is RNase H sensitive. Following overnight immunoprecipitation with the S9.6 antibodies, one wild type sample was treated with recombinant RNase H before the washing steps (see online methods). (c) TERRA levels are not increased in rnh1 rnh201 mutants. qRT-PCR was performed for the 6Y', 1L and 15L telomeres using the indicated mutants (at approx. PD 15 following tetrad dissection). sir2 cells served as positive control where TERRA is upregulated. Average values were derived from 3 biological replicates where error bars depict ± s.e.m.
