Supplementary Figure 3: Rnh1 and Rnh201 act redundantly to remove telomeric RNA-DNA hybrids

(a) The increased rate of senescence shown in Fig. 2c only occurs in absence of telomerase. Senescence curves were performed as previously described (Fig. 1b). The loss of RNase H activity does not result in decreased cell viability in rad52 cells, but accelerates senescence only when both EST2 and RAD52 are co-deleted. Curve averages were derived from 6 biological replicates for each genotype depicted, error bars depict ± s.e.m. est2 rad52 and est2 rad52 rnh1 rnh201 curves are taken form Fig. 2c. (b, c) Senescence curves in an est2 rad52 background were performed with only one of the RNase H genes deleted (rnh1 or rnh201, respectively). Rates of senescence were not affected upon deletion of one RNase H gene only. Curves represent the means of 6 biological replicates ± s.e.m. (d) Raw data from Fig. 2c. Genotypes are indicated.