Supplementary Figure 1: In vitro histone methyltransferase (HMTase) assay.
From: Promiscuous RNA binding by Polycomb repressive complex 2
Assays carried out under identical conditions, except for the exclusion of PRC2, H3.1 or both as highlighted. The higher radioactive signal following TCA precipitation, appearing only in the presence of both PRC2 and H3.1 histone, confirmed HMTase activity of PRC2. Some low signal was observed in the absence of histone substrate, possibly because of loaded SAM that was co-precipitated with PRC2 or a weak auto HMTase activity of PRC2, as previously observed4. Error bars represents standard deviations that were generated based on 3 independent samples.
