Supplementary Figure 4: Structure of the NOT module.
From: Structure and assembly of the NOT module of the human CCR4–NOT complex
(a) Time course of a limited proteolysis of the ternary complex between CNOT1 (residues 1565–2371), CNOT2 (residues 344–540) and CNOT3 (residues 607–753) by thermolysin. CNOT1 is digested into a stable C-terminal fragment corresponding to the SH and an N-terminal fragment (which comigrates with CNOT2), while CNOT2 and CNOT3 remain uncleaved. (b) Time course of a limited proteolysis of CNOT2 (residues 344–540) and CNOT3 (residues 607–753) by thermolysin in the absence or presence of CNOT1 SH (1833–2361). In the absence of CNOT1, CNOT2 and CNOT3 are digested into stable C-terminal fragments corresponding approximately to the NOT-boxes and the CSs, but they remain largely uncleaved in the presence of CNOT1 (c,d) Omit electron density map of the CNOT2 (c) and CNOT3 (d) NAR-Cs folded onto the CNOT1 surface. The electron density (black mesh, 2F0-FC of a composite omit map, calculated with Phenix.AutoBuild) is contoured at 1.0 σ. (e,f) Refined electron density of the CNOT2 (e) and CNOT3 (f) NAR-C regions in stereo view. The views correspond to panels (c) and (d), respectively. The electron density (black mesh, 2F0-FC map) is contoured at 1.0 σ. (g) Superposition of Ct NOT1 (blue) and Hs CNOT1 (gray) showing strong structure conservation. (h) Superposition of the CNOT3 NOT-box domain in isolation (gray) and in the complex (green). (i) Superposition of the NOT-box domains of CNOT2 (purple) and CNOT3 (green) as observed in the ternary complex.
