Supplementary Figure 5: Protein and RNA interactions at the Not boxes.
From: Structure and RNA-binding properties of the Not1–Not2–Not5 module of the yeast Ccr4–Not complex
(a) Surface features of the Not2 and Not5 Not–boxes. On the left is the structure of Not2, showing Arg165 (putative ADA2–binding residue) as well as positively–charged residues at a similar position as in Not5. In the central panel is the Not–box of Not5, in the same orientation, showing the uridine–crosslinked residue Cys546 as well as the surrounding positively–charged residues (as in Fig. 5d). On the right is the structure of RNA–bound SmE (U4 snRNP, PDB code 2Y9A2) oriented in a similar view as the structures in the left and central panels (after optimal superimposition), showing a bound uridine nucleotide (in black). (b) Quantification of the RNA–binding properties of Not1c–Not2–Not5c by fluorescence anisotropy. The Kd of the Not1c–Not2–Not5c complex to 6–FAM–labeled U15 RNA under these conditions was found to be 9.47±0.95 μM.
