Supplementary Figure 1: Identification of the core of the S. cerevisiae Not1-Not2-Not5 interaction.
From: Structure and RNA-binding properties of the Not1–Not2–Not5 module of the yeast Ccr4–Not complex
The 12% SDS PAGE gel shows in lane 1 the larger complex that we initially purified (Not1 starting at residue 1348, Not2 f.l. and Not5 f.l.). Lane 2 shows the result of the limited proteolysis of the complex in lane 1 with elastase. Lane 3 shows the protease alone as a control. Lane 4 shows the complex reconstituted with the minimal interacting regions of Not1, Not2 and Not5 that yielded diffracting crystals (Not1c, Not2 and Not5c).
