Supplementary Figure 4: In vivo interactions of Not proteins.
From: Structure and RNA-binding properties of the Not1–Not2–Not5 module of the yeast Ccr4–Not complex
(a) Immunoprecipitation of Not1 from yeast strains harbouring a TAP tagged Not1 wildtype (WT) or indicated mutants and carrying tagged chromosomal variant of Not2 and Not3 (BSY1230), or Not2 and Pop2/Caf1 (BSY1231). Co–immunoprecipitation of Not2–HA, Not3–VSV or Pop2 VSV was assayed by western blotting. As a control, the presence of the tagged protein in the starting extracts was also assayed. (b) Immunoprecipitation of Not3 from yeast strains carrying tagged chromosomal variant of Not3, Pop2/Caf1 and Not4 (BSY1240), or Not3, Caf1/Pop2 and Not2 (BSY1242). Co-immunoprecipitation of Not2–HA or Not4–HA was assayed by western blotting. As a control, the level of the tagged protein in the starting extracts was also assayed.
