Supplementary Figure 7: Centromere deletion screens of the t(1Rter;3Lter) and Δmsc1 strains.
From: Epigenetically induced paucity of histone H2A.Z stabilizes fission-yeast ectopic centromeres
(a) The viabilities of total cells (blue) and centromere-deleted cells (magenta) in the course of centromere deletion screens (loxP-cen1 and loxP-cen3) of the indicated strains. Error bars represents s.e.m. of n = 6 biological replicates. (b) The ratios of NC formation to telomere fusion in the survivors obtained in each screen. (c) NC mapping of six Δcen3-NC survivors obtained from the t(1Rter;3Lter) loxP-cen3 strain showing the Cnp1 ChIP enrichment values for each strain. All of the Δcen3-NCs were mapped to the region corresponding to Δcen3-NCrDNA (rDNA(L)). (d) NC mapping of six Δcen1-NC survivors obtained from the t(1Rter;3Lter) loxP-cen1 strain. All of the Δcen1-NCs were mapped to the left subtelomeric region of chromosome I (tel1-L) and none were mapped to the right subtelomeric region (tel1-R), even though NCs are formed more preferentially at tel1-R than tel1-L in wild-type cells8.
