Supplementary Figure 5: Relationship between H3K27me3 levels and ezRNA production.
From: PRC2 binds active promoters and contacts nascent RNAs in embryonic stem cells
(a) Correlation analysis between H3K27me3 and EZH2 densities at single ezRNA+ genes (dark gray points, black regression line) or a control set (light gray points, dashed gray regression line) equalized by PRC2 levels as in Fig. 4a (left panel) or RPKMs as in Fig. 4b (right panel). Densities are plotted as log2(RP10M) in each 10 kb window spanning the TSS. (b) Density profiles for H3K27me3 in E14 (left panels) or TNGA (right panels) mouse ESCs grown in serum (top) or 2i (bottom) conditions. The profiles were calculated on the promoters of 1,108 ezRNA+ transcripts (black line) or an equally sized control set of promoters (gray dashed line) matched individually for EZH2 occupancy in E14 cells.
