Supplementary Figure 3: Residues of the model construction basis.
From: Energetic analysis of the rhodopsin–G-protein complex links the α5 helix to GDP release
(a) Residues rebuilt using the Rosetta loop building protocol are shown in magenta coloring. (b) Residues with experimental data are shown in magenta coloring. Regions not rebuilt using the Rosetta loop building protocol are structured as from the template 3sn6, with the exception of the helical domain (green) which undergoes rigid body movements. In (b) DEER distances measured are shown as red lines. (c) Distances measured in Gα by DEER. (d) In black is the model before relaxation, which is compared to the colored model after relaxation. The rmsd between them is 4.1 Å, excluding the helical domain residues 57-180. This shows that the relaxation protocol is a small perturbation protocol.
