Supplementary Figure 4: DEER distributions compared to model and experimental structures.
From: Energetic analysis of the rhodopsin–G-protein complex links the α5 helix to GDP release
(a) Comparison of the experimental distance distribution as observed in EPR DEER measurements (blue) with the predicted distribution computed from the ensemble model of receptor unbound Gαi (red). (b) Comparison of the experimental distance distribution as observed in EPR DEER measurements (blue) with the predicted distribution computed from the ensemble model of the R*-Gi complex (red). In green we show the distance distribution of our previous model which reproduces average distance accurately but not the distance distribution3. In magenta we display the distance distribution based on the crystal structure4.
