Supplementary Figure 6: Characterization experiments of double mutants.
From: Energetic analysis of the rhodopsin–G-protein complex links the α5 helix to GDP release
(a) Binding of doubly spin-labeled mutant G proteins to rhodopsin in disc membranes. (b) Basal (grey) and receptor (black) catalyzed nucleotide exchange rates for the doubly spin-labeled mutant α-subunit. Bars represent the mean of a minimum of three independent experiments, and error bars show standard error of the mean.
